Ground-borne vibrations consistently challenge nearby buildings, impacting their serviceability adversely. This study explores the potential of using sustainable and environment-friendly materials as an effective solution for mitigating such vibrations using bamboo-filled barriers. A series of tests were conducted to investigate the vibration mitigation behavior of both bamboo-filled barriers (BB) and open barriers (OB). Various barrier configurations were used in the current study to strengthen the investigation. The influence of trench location, barrier type, and volume of the filler material on the screening response of trenches is extensively investigated using large-scale tests. In addition, the efficacy of the barrier in mitigating vibrations at measurement locations along the angular lines is explored. The results demonstrate that both barriers exhibit satisfactory performance in vibration mitigation, with their efficacy increasing notably at higher frequencies. Barriers with a lower volume of filler material exhibited superior vibration mitigation compared to those with a higher volume. Furthermore, practical insights into the installation process and precautions for installing BB panels are provided, offering valuable guidance for field engineers in real-world applications.